8.2 Basic Morphometric Analysis

8.2.1 Measuring Length

Figure 8.4: Two Different Kinds of Rulers

Length can be measured using rulers - stage rulers or optical rulers.

The pixel size can then be calculated using:

1. Wide-Field Microscopy

   The pixel size here is fixed after the objective lens is selected.

2. Laser Scanning Confocal Microscopy

   The pixel size here varies on the scanning resolution.

8.2.2 Area and Volume

Figure 8.5: Volume Measurements for 2D and 3D Images

In a 2D image, the area can be calculated as the product of the pixel number and the area of one pixel.

$$\begin{equation} \text{Volume} = \sum_{i = 1}^Si \cdot d \end{equation}$$

Prof. Lu Lei gives the above formula for calculating the area of a 3D sample.