10.3 Cryo Electron Tomography in situ

Figure 10.9: Wang et al.’s Protocol for Cryo EM

Wang et al. lists the following steps for performing cryo electron tomography:

Figure 10.10: ‘Windows’ for Cryo EM

1. Cells are grown on the EM grids.
2. The cells are plunged-freezed in liquid ethane or propane.
3. Cryo FIB milling is done on an Aquilos to make the sample thin enough for TEM.
4. Cryo tomography data is done on the Krios TEM.
5. Data analysis, segmentation, and sub-tomogram averaging is done.

10.3.1 Comparing TEM and Cryo EM

Figure 10.11: Differences Between Cryo EM and TEM

The above slide lists some differences between TEM and CryoEM.